生技種苗檢測服務建置與產業推動
- 種苗研發成果 @ 農業部
計畫名稱_中文生技種苗檢測服務建置與產業推動的主辦人_中文是鍾文全, 主辦單位_中文是生物技術課, 計畫編號_中文是103農科-1.1.2-種-X1, 年度是2014, 中文摘要是利用已開發共顯性分子標誌(Ty1， Ty2及Ty3)，檢測種苗業界所提供的番茄種苗是否具有抗黃化捲葉病毒植株，得知三家種苗業者所育出的番茄種苗主要以抗Ty2為主。文心蘭檸檬綠組培苗依株高等級分為小苗、中苗、大苗等三級，評估小瓶苗要達到出貨標準(株高達7.4cm以上 )約需培養55天，大瓶苗培養30天....
| 年度 | 2014 |
| 計畫名稱_中文 | 生技種苗檢測服務建置與產業推動 |
| 計畫名稱_英文 | Industry extension and establishment of。biotech seedling detection serviceplant seed/seedling team。 |
| 計畫編號_中文 | 103農科-1.1.2-種-X1 |
| 計畫編號_英文 | 103AS -1.1.2-SS-X1 |
| 主辦單位_中文 | 生物技術課 |
| 主辦單位_英文 | Biotechnology Section |
| 主辦人_中文 | 鍾文全 |
| 主辦人_英文 | Wen-Chuan Chung |
| 中文摘要 | 利用已開發共顯性分子標誌(Ty1， Ty2及Ty3)，檢測種苗業界所提供的番茄種苗是否具有抗黃化捲葉病毒植株，得知三家種苗業者所育出的番茄種苗主要以抗Ty2為主。文心蘭檸檬綠組培苗依株高等級分為小苗、中苗、大苗等三級，評估小瓶苗要達到出貨標準(株高達7.4cm以上 )約需培養55天，大瓶苗培養30天即可出貨。拜訪6家仙履蘭組培場，與經營者訪談組培設備、生產流程及瓶苗出貨標準，目前仙履蘭組培瓶苗大多為實生苗，由栽培者提供種子交由專業組培場代工。以栽培場自行雜交之綠Maudiae 品種，無菌播種之實生瓶苗為試驗材料，培養3、4、6個月後，得知仙履蘭瓶苗應以培養4個月後為出瓶最佳時機。本年度蒐集四個流通品種(Phal. TailinRed Angle ‘V31’、Phal. I-Hsin White Swan、Dtps. Tinny Honey、Dtsp. IHsin Yellow Tris)之蝴蝶蘭組培瓶苗，進行生長指數調查與植體營養成分分析，並將蝴蝶蘭瓶苗依102年計畫建立之瓶苗品質鑑定指標進行分級種植，四個流通品種進行瓶苗形態調查及植體分析，並發育到中苗，調查花形間品質指標之結果驗證，結果顯示 5個月後植株之乾鮮重、乾物重、葉幅、葉數、根數等四個品種各級間皆無顯著性差異，若從元素分析來看，各級間元素佔植株乾重的百分比亦並無顯著性差異，但不合苗之單株全可溶性糖含量之表現與合格苗差異較其他元素更為明顯。以去病毒技術篩選蘭園蝴蝶蘭無病毒之母本，共篩選500件瓶苗樣品，結果得知獲得去病毒的比率會隨寄主種類與感染病毒嚴重度有所差異，獲得去病毒的健康蝴蝶蘭種苗比例介於0-15.3%之間。 |
| 英文摘要 | Tomato seedlings provided by seed industry were detected through molecular markers (Ty-1， Ty-2 and Ty-3 developed by TSIP) for identifying TYLCV-ressitant line or variety. Results showed that all tested belong to TY-2 line. Oncidium plants are classified into three levels. To reach standard shipping for small seedling， it could grow up to 55 days，whereas large seedlings only were 30 days. To understand the company profile， production processes and plantlets standards in this year， we visit six paphiopedilum tissue culture factories. Leave number， root number， plant height， root length， fresh/dry weight of paphiopedilum TC。plantlets were studied 3， 4， and 6 months after cultivation. Results showed that 4 months was the best time for shipping. Four circulating cultivars (Phal. Tailin Red Angle 'V31'， Phal.I-Hsin White Swan， Dtps.Tinny Honey， Dtsp. I-Hsin Yellow Tris) of Phalaenopsis were collected from orchid nursery. The Phalaenopsis plantlets， the growth index survey conducted with explants important component of the analysis， and Phalaenopsis plantlets were identified in accordance with quality indicators established 102 years. Four cultivars of circulation were investigated and analyzed after 5 months cultivation. Results showed that growth rate of Phalaenopsis plantlets with 2 roots was not slower than plants with 3 roots after 5 months cultivation. Leaf span， flesh weight，dry weight， leaf number， and root number are not significant differences。in all levels tested. In element analysis， the element concentrations in each level were not significant differences. The total soluble sugar (TSS) and starch of bad plants are significant lower than good plants.Combination of physical method and tissue culture was applied to obtain virus-free Phalaenopsis seedlings. Results showed that percentage of virus-free Phalaenopsis seedlings was varied with the sensitivity of virus and host species， 0-15.3% of virus-free Phalaenopsis seedlings。 |
年度2014 |
計畫名稱_中文生技種苗檢測服務建置與產業推動 |
計畫名稱_英文Industry extension and establishment of。biotech seedling detection serviceplant seed/seedling team。 |
計畫編號_中文103農科-1.1.2-種-X1 |
計畫編號_英文103AS -1.1.2-SS-X1 |
主辦單位_中文生物技術課 |
主辦單位_英文Biotechnology Section |
主辦人_中文鍾文全 |
主辦人_英文Wen-Chuan Chung |
中文摘要利用已開發共顯性分子標誌(Ty1， Ty2及Ty3)，檢測種苗業界所提供的番茄種苗是否具有抗黃化捲葉病毒植株，得知三家種苗業者所育出的番茄種苗主要以抗Ty2為主。文心蘭檸檬綠組培苗依株高等級分為小苗、中苗、大苗等三級，評估小瓶苗要達到出貨標準(株高達7.4cm以上 )約需培養55天，大瓶苗培養30天即可出貨。拜訪6家仙履蘭組培場，與經營者訪談組培設備、生產流程及瓶苗出貨標準，目前仙履蘭組培瓶苗大多為實生苗，由栽培者提供種子交由專業組培場代工。以栽培場自行雜交之綠Maudiae 品種，無菌播種之實生瓶苗為試驗材料，培養3、4、6個月後，得知仙履蘭瓶苗應以培養4個月後為出瓶最佳時機。本年度蒐集四個流通品種(Phal. TailinRed Angle ‘V31’、Phal. I-Hsin White Swan、Dtps. Tinny Honey、Dtsp. IHsin Yellow Tris)之蝴蝶蘭組培瓶苗，進行生長指數調查與植體營養成分分析，並將蝴蝶蘭瓶苗依102年計畫建立之瓶苗品質鑑定指標進行分級種植，四個流通品種進行瓶苗形態調查及植體分析，並發育到中苗，調查花形間品質指標之結果驗證，結果顯示 5個月後植株之乾鮮重、乾物重、葉幅、葉數、根數等四個品種各級間皆無顯著性差異，若從元素分析來看，各級間元素佔植株乾重的百分比亦並無顯著性差異，但不合苗之單株全可溶性糖含量之表現與合格苗差異較其他元素更為明顯。以去病毒技術篩選蘭園蝴蝶蘭無病毒之母本，共篩選500件瓶苗樣品，結果得知獲得去病毒的比率會隨寄主種類與感染病毒嚴重度有所差異，獲得去病毒的健康蝴蝶蘭種苗比例介於0-15.3%之間。 |
英文摘要Tomato seedlings provided by seed industry were detected through molecular markers (Ty-1， Ty-2 and Ty-3 developed by TSIP) for identifying TYLCV-ressitant line or variety. Results showed that all tested belong to TY-2 line. Oncidium plants are classified into three levels. To reach standard shipping for small seedling， it could grow up to 55 days，whereas large seedlings only were 30 days. To understand the company profile， production processes and plantlets standards in this year， we visit six paphiopedilum tissue culture factories. Leave number， root number， plant height， root length， fresh/dry weight of paphiopedilum TC。plantlets were studied 3， 4， and 6 months after cultivation. Results showed that 4 months was the best time for shipping. Four circulating cultivars (Phal. Tailin Red Angle 'V31'， Phal.I-Hsin White Swan， Dtps.Tinny Honey， Dtsp. I-Hsin Yellow Tris) of Phalaenopsis were collected from orchid nursery. The Phalaenopsis plantlets， the growth index survey conducted with explants important component of the analysis， and Phalaenopsis plantlets were identified in accordance with quality indicators established 102 years. Four cultivars of circulation were investigated and analyzed after 5 months cultivation. Results showed that growth rate of Phalaenopsis plantlets with 2 roots was not slower than plants with 3 roots after 5 months cultivation. Leaf span， flesh weight，dry weight， leaf number， and root number are not significant differences。in all levels tested. In element analysis， the element concentrations in each level were not significant differences. The total soluble sugar (TSS) and starch of bad plants are significant lower than good plants.Combination of physical method and tissue culture was applied to obtain virus-free Phalaenopsis seedlings. Results showed that percentage of virus-free Phalaenopsis seedlings was varied with the sensitivity of virus and host species， 0-15.3% of virus-free Phalaenopsis seedlings。 |