1.本計畫嘗試建立流蘇石斛(Dendrobium fimbriatum)之組織培養繁殖技術、種苗生產模式，經試驗顯示流蘇石斛組培苗消毒以使用1%、0.5%次氯酸鈉進行2階段消毒，培植體之存活率最高達50%。芽體增殖階段以培養於DF2培養基2個月，植株之株高可達3.43cm、每芽有4.4片葉、每培殖體形成2.4芽、叢芽率34.68%為最低、培殖體褐化率僅3.00%較佳。發根階段則以培養於1402培養基，可獲得地上部和地下部表現較佳之植株。在種苗生產模式方面，初步探討不同種植芽數、出瓶時間對流蘇石斛組培苗生長之影響，由試驗結果得知，不同種植芽數與出瓶時間對於栽培4個月內之種苗並無顯著之影響。另外，本試驗觀察流蘇石斛在1年的栽培期間之生育變化，將有助於後續調整種苗栽培管理之模式。。2.金童石斛（Dendrobium Cassiope‘Taiseed Golden boy No.1’）為蘭科（Orchidaceae）植物。金童石斛花開花時期為每年的2-4月，我們先前的研究發現金童石斛萃取物有豐富的酚類化合物，且展現優異的抗氧化效果。因此，本研究進一步以脂多醣 (lipopolysaccharide， LPS) 誘發RAW264.7細胞株之發炎模式探討金童石斛乙醇萃取物的抗發炎功效，研究結果發現此萃取物具有抑制RAW264.7細胞發炎之抑制白細胞介素-6 (interleukin-6， IL-6)及腫瘤致死因子(tumor necrosis factor-α， TNF-α)生成，進而抑制一氧化氮 (nitric oxide，NO)和前列腺素E2 (prostagladin E2， PGE2)的生。
英文摘要
1. This study attempts to establish a micropropagation method and seedlingproduction model of Dendrobium fimbriatum， the test showed explants disinfected with 1% and 0.5% sodium hypochlorite twice， the survival rate achieve 50 %. An shoot proliferation medium with code name DF2 was the best for shoot proliferation，the plant height up to 3.43 cm， formed 2.4 buds per explants， and each buds with 4.4 leaves. After two months rooting in the rooting medium with code name 1402， the plantlets can grows well and the rooting rate achieve 100%. In seedling production，we evaluated the effect of different numbers of planting buds and planting timing on the growth of Dendrobium fimbriatum plantlets.Results showed that the different number of planting buds and planting timing have no significant influence on seedling growth in four months test. In addition， we observation the cultivation increment changes of seedling in the period of a year， will help to adjust the method of seedling cultivation and management of Dendrobium fimbriatum.。2. Dendrobium Cassiope‘Taiseed Golden boy No.1’ belonging to Orchidaceae family is one of the new medicinal dendrobium in Taiwan， which blooms in late february and fruit matures in late April. Our pervious study found that D. Cassiope stem extract had a notable amount of phenol and also exhibited good antioxidant capacity. In this study， antiinflammation effect of ethanol extract of D. Cassiope stem was evaluated with lipopolysaccharide (LPS)-stimulated RAW264.7 cell lines further. Results show that the ethanol extract could reduce interleukin-6 (IL-6) and ， tumor necrosis factor-α(TNF-α)release， and lower nitric oxide (NO) and prostaglandin E2 (PGE2) secretion in LPSactivated RAW264.7 cells.。
1.本計畫嘗試建立流蘇石斛(Dendrobium fimbriatum)之組織培養繁殖技術、種苗生產模式，經試驗顯示流蘇石斛組培苗消毒以使用1%、0.5%次氯酸鈉進行2階段消毒，培植體之存活率最高達50%。芽體增殖階段以培養於DF2培養基2個月，植株之株高可達3.43cm、每芽有4.4片葉、每培殖體形成2.4芽、叢芽率34.68%為最低、培殖體褐化率僅3.00%較佳。發根階段則以培養於1402培養基，可獲得地上部和地下部表現較佳之植株。在種苗生產模式方面，初步探討不同種植芽數、出瓶時間對流蘇石斛組培苗生長之影響，由試驗結果得知，不同種植芽數與出瓶時間對於栽培4個月內之種苗並無顯著之影響。另外，本試驗觀察流蘇石斛在1年的栽培期間之生育變化，將有助於後續調整種苗栽培管理之模式。。2.金童石斛（Dendrobium Cassiope‘Taiseed Golden boy No.1’）為蘭科（Orchidaceae）植物。金童石斛花開花時期為每年的2-4月，我們先前的研究發現金童石斛萃取物有豐富的酚類化合物，且展現優異的抗氧化效果。因此，本研究進一步以脂多醣 (lipopolysaccharide， LPS) 誘發RAW264.7細胞株之發炎模式探討金童石斛乙醇萃取物的抗發炎功效，研究結果發現此萃取物具有抑制RAW264.7細胞發炎之抑制白細胞介素-6 (interleukin-6， IL-6)及腫瘤致死因子(tumor necrosis factor-α， TNF-α)生成，進而抑制一氧化氮 (nitric oxide，NO)和前列腺素E2 (prostagladin E2， PGE2)的生。
英文摘要
1. This study attempts to establish a micropropagation method and seedlingproduction model of Dendrobium fimbriatum， the test showed explants disinfected with 1% and 0.5% sodium hypochlorite twice， the survival rate achieve 50 %. An shoot proliferation medium with code name DF2 was the best for shoot proliferation，the plant height up to 3.43 cm， formed 2.4 buds per explants， and each buds with 4.4 leaves. After two months rooting in the rooting medium with code name 1402， the plantlets can grows well and the rooting rate achieve 100%. In seedling production，we evaluated the effect of different numbers of planting buds and planting timing on the growth of Dendrobium fimbriatum plantlets.Results showed that the different number of planting buds and planting timing have no significant influence on seedling growth in four months test. In addition， we observation the cultivation increment changes of seedling in the period of a year， will help to adjust the method of seedling cultivation and management of Dendrobium fimbriatum.。2. Dendrobium Cassiope‘Taiseed Golden boy No.1’ belonging to Orchidaceae family is one of the new medicinal dendrobium in Taiwan， which blooms in late february and fruit matures in late April. Our pervious study found that D. Cassiope stem extract had a notable amount of phenol and also exhibited good antioxidant capacity. In this study， antiinflammation effect of ethanol extract of D. Cassiope stem was evaluated with lipopolysaccharide (LPS)-stimulated RAW264.7 cell lines further. Results show that the ethanol extract could reduce interleukin-6 (IL-6) and ， tumor necrosis factor-α(TNF-α)release， and lower nitric oxide (NO) and prostaglandin E2 (PGE2) secretion in LPSactivated RAW264.7 cells.。