一、健康種苗之母本檢測與母本園建構及維護：。本年度完成馬鈴薯12品種(系)及鳳梨3品種病毒檢測，所測樣品皆無血清反應。並完成母本園設施改善與建置，可供健康種苗母本保存及檢測研發利用。另外，利用實驗室可取得之8種不同材質吸附膜，進行馬鈴薯病毒 Y 之汁液印漬捕捉反轉錄聚合酶鏈鎖反應(Print Capture RT-PCR， PC-RT-PCR)檢測技術測試，以建立可縮短抽取核酸的人力物力之檢測技術，試驗結果以CALBIOCHEM公司產品Miracloth為最佳。。二、ISTA豆類種子健康檢查驗證體系之建立：。為建立種子檢查室之豌豆葉斑病菌與菜豆炭疽病菌檢測作業流程，本年度依國際種子檢查協會公告之檢測方式建立檢測流程，並實際測試檢測流程的穩定性。。三、種子(苗)品質純度分子檢測技術研發及標準化：。本年度以葫蘆科西瓜為基礎，開發20組SSR與16組SNP品種檢定分子標誌，經篩選後計有14組SSR與13組SNP標誌可檢定計22個不同的西瓜雜交組合，且計有至少93個多型性表現。。
英文摘要
In this study， virus diseases in 12 varieties of potato and 3 varieties of pineapple were tested， and all samples would have produced negative results. In addition， facilities of mother-stock house had been improved，that would benefit healthy seedling conservation and development. The 8 different kinds absorbent membranes were test to instead the RNA extraction protocol before RT-PCR for PVY detection. The most efficient material was Miracloth which was production by CALBIOCHEM. For establishment the health check of the seed beans verification system by ISTA， the objective of this program is to establish the seed testing procedures on Ascochyta pinodes and Colletotrichum lindemuthianum. The procedure in coincidence with ISTA rules has been established and confirmed the stability. To increase industrial competitiveness， 20 SSR and 16 SNP markers had been developed in this project for testing hybridization seeds based on cucurbitaceae. Results showed that 14 SSR and 13 SNP markers distinguish the hybridization purity in 22 watermelon hybrids and represent at least 93 polymorfics.
一、健康種苗之母本檢測與母本園建構及維護：。本年度完成馬鈴薯12品種(系)及鳳梨3品種病毒檢測，所測樣品皆無血清反應。並完成母本園設施改善與建置，可供健康種苗母本保存及檢測研發利用。另外，利用實驗室可取得之8種不同材質吸附膜，進行馬鈴薯病毒 Y 之汁液印漬捕捉反轉錄聚合酶鏈鎖反應(Print Capture RT-PCR， PC-RT-PCR)檢測技術測試，以建立可縮短抽取核酸的人力物力之檢測技術，試驗結果以CALBIOCHEM公司產品Miracloth為最佳。。二、ISTA豆類種子健康檢查驗證體系之建立：。為建立種子檢查室之豌豆葉斑病菌與菜豆炭疽病菌檢測作業流程，本年度依國際種子檢查協會公告之檢測方式建立檢測流程，並實際測試檢測流程的穩定性。。三、種子(苗)品質純度分子檢測技術研發及標準化：。本年度以葫蘆科西瓜為基礎，開發20組SSR與16組SNP品種檢定分子標誌，經篩選後計有14組SSR與13組SNP標誌可檢定計22個不同的西瓜雜交組合，且計有至少93個多型性表現。。
英文摘要
In this study， virus diseases in 12 varieties of potato and 3 varieties of pineapple were tested， and all samples would have produced negative results. In addition， facilities of mother-stock house had been improved，that would benefit healthy seedling conservation and development. The 8 different kinds absorbent membranes were test to instead the RNA extraction protocol before RT-PCR for PVY detection. The most efficient material was Miracloth which was production by CALBIOCHEM. For establishment the health check of the seed beans verification system by ISTA， the objective of this program is to establish the seed testing procedures on Ascochyta pinodes and Colletotrichum lindemuthianum. The procedure in coincidence with ISTA rules has been established and confirmed the stability. To increase industrial competitiveness， 20 SSR and 16 SNP markers had been developed in this project for testing hybridization seeds based on cucurbitaceae. Results showed that 14 SSR and 13 SNP markers distinguish the hybridization purity in 22 watermelon hybrids and represent at least 93 polymorfics.