Multiplex PCR and PCR was apllied to detect GM papaya seedlings.Multiplex PCR had the same result as general PCR， resulting in cost reduction and enhancing detection capacity. We visit six Oncidium tissue culture factories， to understand the company profile， production processes and plantlets standards in this year. Furthermore， we gather commercially Oncidium TC plantlets to explore the influence of the external traits of plantlets quality， including the number of leaves，root number， plant height， root length， fresh/dry weight. Eight hundred and ten young plantlets were found no significant difference between the number of leaves， root number and root length were have a higher degree of variation， presumably by sub-culture transplant operation. Plant height is still relatively easy and reliable grading standards.Preliminary results suggest that large and medium grades are ready for marketing and management model of the small grade plantlets need further evaluation. On the other hand， we also visited 16 Phalaenopsis tissue culture factories and interviewed with the operators about TC equipment，production processes and plantlets standard shipping. We collected 12 portion of Phalaenopsis Sogo Yukidiam ‘V3’ flasks from 11 TC factories，each with 6 flasks， a total of 72 samples. These in vitro plantlets were followed by qualified survey， calculating the numbers and weights of shoots and roots and then divided into four quality levels. We also took some samples to analyze the implant component， and the remaining plants were moved in a professional orchid nursery until they grew to medium size. The medium plants were also measured and analyzed the same item as in vitro plantlets. These medium plants were classified to four grades according to the analysis. Superior Phalaenopsis plantlets have upright type， normal leaf and root shapes， more than three leaves， two or more valid roots and plant weight is higher than 3 grams. These plants also have moderated leaf nitrogen content (1.5-4% dry weight)， high levels of potassium (more than 5% dry weight). 04-0.8% phosphorus dry weight content， the calcium content of less than 0.4% and the magnesium content 0.15% or more.1346 samples of Phalaenopsis tissue culture(TC) seedling collected from 3 orchid nurseries were detected by RT-PCR technique for Odontoglossum ringspot virus(ORSV)and Cymbidium mosaic virus(CymMV).Results showed that 92.8% samples were healthy seedlings
Multiplex PCR and PCR was apllied to detect GM papaya seedlings.Multiplex PCR had the same result as general PCR， resulting in cost reduction and enhancing detection capacity. We visit six Oncidium tissue culture factories， to understand the company profile， production processes and plantlets standards in this year. Furthermore， we gather commercially Oncidium TC plantlets to explore the influence of the external traits of plantlets quality， including the number of leaves，root number， plant height， root length， fresh/dry weight. Eight hundred and ten young plantlets were found no significant difference between the number of leaves， root number and root length were have a higher degree of variation， presumably by sub-culture transplant operation. Plant height is still relatively easy and reliable grading standards.Preliminary results suggest that large and medium grades are ready for marketing and management model of the small grade plantlets need further evaluation. On the other hand， we also visited 16 Phalaenopsis tissue culture factories and interviewed with the operators about TC equipment，production processes and plantlets standard shipping. We collected 12 portion of Phalaenopsis Sogo Yukidiam ‘V3’ flasks from 11 TC factories，each with 6 flasks， a total of 72 samples. These in vitro plantlets were followed by qualified survey， calculating the numbers and weights of shoots and roots and then divided into four quality levels. We also took some samples to analyze the implant component， and the remaining plants were moved in a professional orchid nursery until they grew to medium size. The medium plants were also measured and analyzed the same item as in vitro plantlets. These medium plants were classified to four grades according to the analysis. Superior Phalaenopsis plantlets have upright type， normal leaf and root shapes， more than three leaves， two or more valid roots and plant weight is higher than 3 grams. These plants also have moderated leaf nitrogen content (1.5-4% dry weight)， high levels of potassium (more than 5% dry weight). 04-0.8% phosphorus dry weight content， the calcium content of less than 0.4% and the magnesium content 0.15% or more.1346 samples of Phalaenopsis tissue culture(TC) seedling collected from 3 orchid nurseries were detected by RT-PCR technique for Odontoglossum ringspot virus(ORSV)and Cymbidium mosaic virus(CymMV).Results showed that 92.8% samples were healthy seedlings