The projects of this program have three components including. The first is the establishment and application molecular markers of the resistance to Fusarium oxysporum in tomato analysis system. Specific markers I2 was developed for differentiating allelic variation at I-2 locus. It amplifies fragments from both resistant and susceptible tomato accessions with different molecular sizes that corresponding to the presence of I-2/I-2， I-2/i-2 and i-2/i-2 alleles. This study we also merged primer set I2/Fu as Multiplex-PCR through once PCR， detecting I-2， i-2 andFusarium oxysporum simultaneously. Primer I2/Fu could amplify resistance genes I-2。0.7 or 0.8 kb， susceptibility gene i-2 2.2 kb and Fusarium spp. 1.0 kb of DNA bands. The molecular markers could help breeders early screening and confirmation disease resistant plant genotypes， helping to accelerate the breeding and promotion of disease-resistant tomato lines.。Using seven cauliflower lines which are commonly used breeding parental as test material. To do SCAR primers screening based on Brace’s PCRrestriction classification test results that established in 1994. The result of PCR-restriction classification in addition to the line SP04 and SP06 are same self-incompatibility genotype， the other are different.Then the SCAR makers screening results showed that there are two sets of SCAR primers: D4-1 * D7-2 can identify lines SP01， SP04/SP06， SP08； K1-2 * K4-4 can identify lines SP07， SP08， SP09， SP10.。Tobacco mosaic virus (TMV) is a serious constraint to the production，often causing 100% yield loss. In the world， the disese is caused by three strains， TMV-0， TMV-1， TMV-2. The main disease resistance genes identified include Tm-1， Tm-2 and Tm-2 2. In this study， we have developed the method of molecular markers with AVRDC for specific detection of TMV，and molecular markers for resistance genes Tm-2. The results showed we has been established molecular marker， TMV， could be amplified tobacco mosaic virus 2.2 kb of DNA band. Primer Tm2 could amplify resistance genes Tm-2 650 bp and susceptibility gene tm-2 450 bp of DNA bands. The molecular markers could help breeders early screening and confirmation disease resistant plant genotypes (R / R， R / S， S / S)， helping to accelerate the breeding and promotion of disease-resistant tomato lines.。
The projects of this program have three components including. The first is the establishment and application molecular markers of the resistance to Fusarium oxysporum in tomato analysis system. Specific markers I2 was developed for differentiating allelic variation at I-2 locus. It amplifies fragments from both resistant and susceptible tomato accessions with different molecular sizes that corresponding to the presence of I-2/I-2， I-2/i-2 and i-2/i-2 alleles. This study we also merged primer set I2/Fu as Multiplex-PCR through once PCR， detecting I-2， i-2 andFusarium oxysporum simultaneously. Primer I2/Fu could amplify resistance genes I-2。0.7 or 0.8 kb， susceptibility gene i-2 2.2 kb and Fusarium spp. 1.0 kb of DNA bands. The molecular markers could help breeders early screening and confirmation disease resistant plant genotypes， helping to accelerate the breeding and promotion of disease-resistant tomato lines.。Using seven cauliflower lines which are commonly used breeding parental as test material. To do SCAR primers screening based on Brace’s PCRrestriction classification test results that established in 1994. The result of PCR-restriction classification in addition to the line SP04 and SP06 are same self-incompatibility genotype， the other are different.Then the SCAR makers screening results showed that there are two sets of SCAR primers: D4-1 * D7-2 can identify lines SP01， SP04/SP06， SP08； K1-2 * K4-4 can identify lines SP07， SP08， SP09， SP10.。Tobacco mosaic virus (TMV) is a serious constraint to the production，often causing 100% yield loss. In the world， the disese is caused by three strains， TMV-0， TMV-1， TMV-2. The main disease resistance genes identified include Tm-1， Tm-2 and Tm-2 2. In this study， we have developed the method of molecular markers with AVRDC for specific detection of TMV，and molecular markers for resistance genes Tm-2. The results showed we has been established molecular marker， TMV， could be amplified tobacco mosaic virus 2.2 kb of DNA band. Primer Tm2 could amplify resistance genes Tm-2 650 bp and susceptibility gene tm-2 450 bp of DNA bands. The molecular markers could help breeders early screening and confirmation disease resistant plant genotypes (R / R， R / S， S / S)， helping to accelerate the breeding and promotion of disease-resistant tomato lines.。