(a) 重要蘭科花卉品種分子標誌技術開發：。本年度透過委辦計畫與成功大學生物科學系吳副教授文鑾， 針對蝴蝶蘭（Phalaenopsis spp.）商業品種SSR分子標誌鑑定系統，共同建立一套包含十組SSR分子標誌之試驗方法，並制定標準試驗流程，也選定Dtps. Leopard Prince ‘世芥鑽石F-1138’與P. Zuma’s Pixie ‘台大小可愛’兩個蝴蝶蘭商業品種作為標準試驗流程之參考品種。依據已制定之標準試驗流程，鑑定100個已於台灣取得植物品種權蝴蝶蘭商業品種之基因型並以商業軟體建立DNA資料庫，十組SSR分子標誌可完全鑑別100個蝴蝶蘭商業品種，其PIC (polymorphism information content)值介於0.716 (PHS03)與0.893 (PHS07)之間，平均約0.84。並進行多方實驗室之能力試測試與12個蝴蝶蘭商業品種的盲樣試驗，確認所建立標準試驗流程之結果可再現性與穩定性，顯示此套蝴蝶蘭SSR分子標誌品種鑑定系統可有效率協助鑑別蝴蝶蘭品種與國際間相關技術之應用交流。。(b) 檢定人員訓練及植物品種權教育推廣：。作物新品種檢定講習會於102年10月30日已辦理訓練講習，上課學員約計60人，本次課程內容有植物品種權申請與審查規範介紹、蘭花、蔬菜及糧食作物性狀開發介紹，另外並加入了蝴蝶蘭及黛粉葉檢定技術實習課程，讓上課的學員實際參與檢定技術的操作，實習課程充實，學員熱烈參與。。
英文摘要
(a) To develop the molecular marker technology of important orchid。flower varieties:。To cooperate with Dr. Wu from NCKU through the commissioned project to establish the standard operation procedure (SOP) of Phalaenopsis variety identification comprising ten SSR markers and two Phalaenopsis varieties，Dtps. Leopard Prince ‘Sogo F-1138’ and P. Zuma’s Pixie ‘Taida Little Cutie’， were selected as reference varieties of this identification system. The genotypes of 100 Phalaenopsis varieties granted PBR in Taiwan were analyzed based on the established SOP and all these 100 PBR varieties could be discriminated by the ten SSR markers. The PIC (polymorphism information content) values of the SSR markers ranged from 0.716 (PHS03) to 0.893 (PHS07)， in average of 0.84. The multi-test and blind tests on 12 Phalaenopsis varieties were also conducted to confirm the data reproducibility and stability of this Phalaenopsis variety identification system. These results revealed that the Phalaenopsis variety identification system based on SSR markers can effectively discriminate Phalaenopsis varieties and accelerate the cooperation of using this applicable technique with other international organizations.。(b)Personnel training and promotion of plant variety rights education:。New crop varieties DUS testing workshop has been held on 30 October 2013.There are about 60 students in this training class. The curriculum included that application of plant variety rights， standard of application form and developed DUS testing of orchids， vegetables and grain crops. In addition， the operation trait program of Phalaenopsis and Dieffenbachia’s DUS testing technology which will let participators to know the relative technologies of DUS testing and system of plant variety protection. The outcomes of this program will provide helpfully information of plant variety protection and enhance credibility of molecular testing system.。
(a) 重要蘭科花卉品種分子標誌技術開發：。本年度透過委辦計畫與成功大學生物科學系吳副教授文鑾， 針對蝴蝶蘭（Phalaenopsis spp.）商業品種SSR分子標誌鑑定系統，共同建立一套包含十組SSR分子標誌之試驗方法，並制定標準試驗流程，也選定Dtps. Leopard Prince ‘世芥鑽石F-1138’與P. Zuma’s Pixie ‘台大小可愛’兩個蝴蝶蘭商業品種作為標準試驗流程之參考品種。依據已制定之標準試驗流程，鑑定100個已於台灣取得植物品種權蝴蝶蘭商業品種之基因型並以商業軟體建立DNA資料庫，十組SSR分子標誌可完全鑑別100個蝴蝶蘭商業品種，其PIC (polymorphism information content)值介於0.716 (PHS03)與0.893 (PHS07)之間，平均約0.84。並進行多方實驗室之能力試測試與12個蝴蝶蘭商業品種的盲樣試驗，確認所建立標準試驗流程之結果可再現性與穩定性，顯示此套蝴蝶蘭SSR分子標誌品種鑑定系統可有效率協助鑑別蝴蝶蘭品種與國際間相關技術之應用交流。。(b) 檢定人員訓練及植物品種權教育推廣：。作物新品種檢定講習會於102年10月30日已辦理訓練講習，上課學員約計60人，本次課程內容有植物品種權申請與審查規範介紹、蘭花、蔬菜及糧食作物性狀開發介紹，另外並加入了蝴蝶蘭及黛粉葉檢定技術實習課程，讓上課的學員實際參與檢定技術的操作，實習課程充實，學員熱烈參與。。
英文摘要
(a) To develop the molecular marker technology of important orchid。flower varieties:。To cooperate with Dr. Wu from NCKU through the commissioned project to establish the standard operation procedure (SOP) of Phalaenopsis variety identification comprising ten SSR markers and two Phalaenopsis varieties，Dtps. Leopard Prince ‘Sogo F-1138’ and P. Zuma’s Pixie ‘Taida Little Cutie’， were selected as reference varieties of this identification system. The genotypes of 100 Phalaenopsis varieties granted PBR in Taiwan were analyzed based on the established SOP and all these 100 PBR varieties could be discriminated by the ten SSR markers. The PIC (polymorphism information content) values of the SSR markers ranged from 0.716 (PHS03) to 0.893 (PHS07)， in average of 0.84. The multi-test and blind tests on 12 Phalaenopsis varieties were also conducted to confirm the data reproducibility and stability of this Phalaenopsis variety identification system. These results revealed that the Phalaenopsis variety identification system based on SSR markers can effectively discriminate Phalaenopsis varieties and accelerate the cooperation of using this applicable technique with other international organizations.。(b)Personnel training and promotion of plant variety rights education:。New crop varieties DUS testing workshop has been held on 30 October 2013.There are about 60 students in this training class. The curriculum included that application of plant variety rights， standard of application form and developed DUS testing of orchids， vegetables and grain crops. In addition， the operation trait program of Phalaenopsis and Dieffenbachia’s DUS testing technology which will let participators to know the relative technologies of DUS testing and system of plant variety protection. The outcomes of this program will provide helpfully information of plant variety protection and enhance credibility of molecular testing system.。