Virus and pathogen detection and establishment of mother-stock garden is the fundamental of healthy seedling production. In this program， we will implement the developing pathogen validation system and construction mother-stock garden to maintain specific pathogen free mother-stock such as potato， grapes， strawberry and bamboo etc. Our results indicated grapes， strawberry and bamboo kept in mother-stock garden were not infected by specific pathogens. Potato stocks were infected by potato virus S or potato virus Y and using none certification potato seed tube would face higher infection risk. We hope this effort could support healthy seedling production in Taiwan.。To refer to the scientific or technical literature， we completed the detection technology inventory of Cucurbit chlorotic yellows virus， CCYV and Strawberry latent ringspot virus， SLRSV. The main detection technologies of CCYV are RT-PCR，Western blotting，ELISA and Immunoelectron microscopy. The main detection technologies of SLRSV are RT-PCR， IC-RT-PCR， ELISA and pathognomy. By taking different samples to test the detection technology， we choose RT-PCR for CCYV and ELISA for SLRSV to be the most suitable detectoion method. And we establish standard testing process (SOP)， and finish the ability to test.。The objective of this program is to establish the seed testing procedure on Xanthomonas campestris pv. campestris (Xcc) which causes black rot of crucifers. The procedure in coincidence with ISTA rules has been established. Artificial infested seeds were adopted to check the Xcc testing procedure. Xcc showed pale green and pale yellow mucoid colonies individually on FS and mCS20ABN selective media. And the Xcc-carried ratio of 0.01% could be detected in sensitivity test. No Xcc was detected in 7 lots of crucifer seeds from different producers. The documents for。quality management had also been completed.。One required index of high quality of seed/seedling is genetic purity，especially for hybrid seed. Genetic purity in a commercial hybrid of solanaceous crops is very important. This year， we setup this quality control mode by using molecular markers in seed production system of the crops of tomato and pepper， last two year we did some part work in tomato and pepper. The year， We obtain six new sets of SCAR primer for three specific DNA fragments in tomato and ten new sets of SCAR primer for six specific DNA fragments in pepper， they can identify hybrid from parents.The SCAR primer can have the sharp and easy-read DNA fragment after PCR amplification and electrophoresis. We will collect these SCAR primers as members of data base for testing F1 hybrid seed purity in tomato and pepper.。
Virus and pathogen detection and establishment of mother-stock garden is the fundamental of healthy seedling production. In this program， we will implement the developing pathogen validation system and construction mother-stock garden to maintain specific pathogen free mother-stock such as potato， grapes， strawberry and bamboo etc. Our results indicated grapes， strawberry and bamboo kept in mother-stock garden were not infected by specific pathogens. Potato stocks were infected by potato virus S or potato virus Y and using none certification potato seed tube would face higher infection risk. We hope this effort could support healthy seedling production in Taiwan.。To refer to the scientific or technical literature， we completed the detection technology inventory of Cucurbit chlorotic yellows virus， CCYV and Strawberry latent ringspot virus， SLRSV. The main detection technologies of CCYV are RT-PCR，Western blotting，ELISA and Immunoelectron microscopy. The main detection technologies of SLRSV are RT-PCR， IC-RT-PCR， ELISA and pathognomy. By taking different samples to test the detection technology， we choose RT-PCR for CCYV and ELISA for SLRSV to be the most suitable detectoion method. And we establish standard testing process (SOP)， and finish the ability to test.。The objective of this program is to establish the seed testing procedure on Xanthomonas campestris pv. campestris (Xcc) which causes black rot of crucifers. The procedure in coincidence with ISTA rules has been established. Artificial infested seeds were adopted to check the Xcc testing procedure. Xcc showed pale green and pale yellow mucoid colonies individually on FS and mCS20ABN selective media. And the Xcc-carried ratio of 0.01% could be detected in sensitivity test. No Xcc was detected in 7 lots of crucifer seeds from different producers. The documents for。quality management had also been completed.。One required index of high quality of seed/seedling is genetic purity，especially for hybrid seed. Genetic purity in a commercial hybrid of solanaceous crops is very important. This year， we setup this quality control mode by using molecular markers in seed production system of the crops of tomato and pepper， last two year we did some part work in tomato and pepper. The year， We obtain six new sets of SCAR primer for three specific DNA fragments in tomato and ten new sets of SCAR primer for six specific DNA fragments in pepper， they can identify hybrid from parents.The SCAR primer can have the sharp and easy-read DNA fragment after PCR amplification and electrophoresis. We will collect these SCAR primers as members of data base for testing F1 hybrid seed purity in tomato and pepper.。