Twenty-seven different pumpkin hybrid lines were screened to the powdery mildew -resistance which inoculum pathogen species. According the inoculum results showed that the virulence were different by the host hybrid lines. The hybrid lines were almost easy to infect by the powdery mildew in these year. We construct a grade of the powdery mildew –resistance in pumpkin.。By 16S rDNA sequences analysis， antagonists No. 14-12 and 15-25 which were selected in 2012 were identified as Bacillus megaterium. We have tested 11 media formulas， yet non of them had the effect to make the cell concentration of antagonists No. 14-12 and 15-25 up to 109/ml. However，according to the results， it was found that adding suitable calcium salt could promote cell growth.。Before enter a large number of plant tissue culture propagation， to detect the plant target disease can ensure a healthy production procedure. The specific pathogen free tissue culture units also are the cornerstone for the production of healthy seedlings. Tissue culture is the subject diseases including viral diseases， viral diseases and class mycoplasma disease. And techniques for detect these pathogen are enzymelinked immuno-sorbent assay， ELISA and Reverse transcription-PCR， RT-PCR.The cost of serum is the highest value of ELISA. This project is intended to express recombinant proteins in transgenic E. coli， the subject of the virus as a source of protein production， the production of important crops of the serum， the plans to complete the production of potato virus PVS， antiserum PVX， PVY of the production each 80ml， diluted potency up to 8000-16，000 times。
Twenty-seven different pumpkin hybrid lines were screened to the powdery mildew -resistance which inoculum pathogen species. According the inoculum results showed that the virulence were different by the host hybrid lines. The hybrid lines were almost easy to infect by the powdery mildew in these year. We construct a grade of the powdery mildew –resistance in pumpkin.。By 16S rDNA sequences analysis， antagonists No. 14-12 and 15-25 which were selected in 2012 were identified as Bacillus megaterium. We have tested 11 media formulas， yet non of them had the effect to make the cell concentration of antagonists No. 14-12 and 15-25 up to 109/ml. However，according to the results， it was found that adding suitable calcium salt could promote cell growth.。Before enter a large number of plant tissue culture propagation， to detect the plant target disease can ensure a healthy production procedure. The specific pathogen free tissue culture units also are the cornerstone for the production of healthy seedlings. Tissue culture is the subject diseases including viral diseases， viral diseases and class mycoplasma disease. And techniques for detect these pathogen are enzymelinked immuno-sorbent assay， ELISA and Reverse transcription-PCR， RT-PCR.The cost of serum is the highest value of ELISA. This project is intended to express recombinant proteins in transgenic E. coli， the subject of the virus as a source of protein production， the production of important crops of the serum， the plans to complete the production of potato virus PVS， antiserum PVX， PVY of the production each 80ml， diluted potency up to 8000-16，000 times。