The increasing presence of transgenic plant in the international markets has provoked a strong demand for appropriate detection methods to evaluate the existence of transgenic plants. Technique for detection of GM crops consists of four distinct steps， including detection capability，proficiency test， the standard detection and monitoring model. The biological area of detection laboratory at SIPS has been accredited according to ISO/IEC 17025. There are several items to be accredited by TAF， including transgenic papaya (Papaya ring spot virus coat protein gene， prsv-cp gene； Papaya ring spot virus coat protein gene， PRSV CP gene；papaya leaf distortion mosaic virus， PLDMV～py16-cp gene)， maize (TC1507， BT-176， BT11， MON810， NK603， 3272， MIR604， GA21， 59122， MON863) and soybean (GTS 40-3-2， DP-305423-1， DP-356043-5). The major objective of this study was to establish a rapid detection technique for the GM maize and soybean. For this purpose， some screening methods based on target gene and select marker was development. The results showed that the GM crops could be detected through Real-time PCR rapidly and the R2 above 0.9.
The increasing presence of transgenic plant in the international markets has provoked a strong demand for appropriate detection methods to evaluate the existence of transgenic plants. Technique for detection of GM crops consists of four distinct steps， including detection capability，proficiency test， the standard detection and monitoring model. The biological area of detection laboratory at SIPS has been accredited according to ISO/IEC 17025. There are several items to be accredited by TAF， including transgenic papaya (Papaya ring spot virus coat protein gene， prsv-cp gene； Papaya ring spot virus coat protein gene， PRSV CP gene；papaya leaf distortion mosaic virus， PLDMV～py16-cp gene)， maize (TC1507， BT-176， BT11， MON810， NK603， 3272， MIR604， GA21， 59122， MON863) and soybean (GTS 40-3-2， DP-305423-1， DP-356043-5). The major objective of this study was to establish a rapid detection technique for the GM maize and soybean. For this purpose， some screening methods based on target gene and select marker was development. The results showed that the GM crops could be detected through Real-time PCR rapidly and the R2 above 0.9.