An efficient in vitro propagation protocol for nobile-type dendrobium was established. In this study， a shoot proliferation medium supplemented with different concentration of cytokinins (BA or TDZ) was evaluated its effect on shoot proliferation. The results indicated that using shoot proliferation medium B4 or T2 were optimal for shoot proliferation with shoot proliferation number per explant of 3.12， 3.23. The rooting rate of plantlets were 100% after two months rooting in the rooting medium –N1B3， the average root number and root length of plantlets were 5.6 and 4.1 cm， respectively. In addition， the purpose of this study was to evaluate the effect of fertilizers with different rate of nitrogen (N)， phosphorus (P)， and potassium (K) on the growth and flowering of the nobile-type dendrobium. The results show that treatments of N1-P1-K1 and N1-P2-K1 were optimal for growth， but the N1-P2-K2 treatment was optimal for flowering.。We have visited 2 Dendrobium entertainments， confirming the main。diseases are anthracnose and Pseudocercospra leaf spots. By sampling diseased plants and tissue separating， 4 strains of Colletotrichum spp.were obtained. Leaves of 5 Dendrobium varieties were inoculated with these strains for making sure their pathogenicity.The objectives of the present study were to develop culture method for shoot formation in 3cultivars of pineapple. Treatment of 0.5 mg/L TDZ was found best in callus induction from lateral bud explants， induction percentage reached 80% ， shoots were formed after culture for 3 weeks.The highest percent shoot formation which is 98% was found in the treatment of 3.0 mg/L BA. In addition， treatment of 2.0 mg/L NAA or IAAwas found best in root formation from shoot， formation percentage reached 95% ， the average roots formed per shoot were 2.1。。A water-soluble crude extracts was obtained from Dendrobium Taiseed Tosnobile by cold water extraction(CWDTT). The data was showed the activities to stimulate macrophages and enhanced the release of NO from RAW264.7 macrophages. Furthermore， the concentration of Interleukin-6(IL-6) and TNF-α were significantly stimulated after exposed to CWDTT。
An efficient in vitro propagation protocol for nobile-type dendrobium was established. In this study， a shoot proliferation medium supplemented with different concentration of cytokinins (BA or TDZ) was evaluated its effect on shoot proliferation. The results indicated that using shoot proliferation medium B4 or T2 were optimal for shoot proliferation with shoot proliferation number per explant of 3.12， 3.23. The rooting rate of plantlets were 100% after two months rooting in the rooting medium –N1B3， the average root number and root length of plantlets were 5.6 and 4.1 cm， respectively. In addition， the purpose of this study was to evaluate the effect of fertilizers with different rate of nitrogen (N)， phosphorus (P)， and potassium (K) on the growth and flowering of the nobile-type dendrobium. The results show that treatments of N1-P1-K1 and N1-P2-K1 were optimal for growth， but the N1-P2-K2 treatment was optimal for flowering.。We have visited 2 Dendrobium entertainments， confirming the main。diseases are anthracnose and Pseudocercospra leaf spots. By sampling diseased plants and tissue separating， 4 strains of Colletotrichum spp.were obtained. Leaves of 5 Dendrobium varieties were inoculated with these strains for making sure their pathogenicity.The objectives of the present study were to develop culture method for shoot formation in 3cultivars of pineapple. Treatment of 0.5 mg/L TDZ was found best in callus induction from lateral bud explants， induction percentage reached 80% ， shoots were formed after culture for 3 weeks.The highest percent shoot formation which is 98% was found in the treatment of 3.0 mg/L BA. In addition， treatment of 2.0 mg/L NAA or IAAwas found best in root formation from shoot， formation percentage reached 95% ， the average roots formed per shoot were 2.1。。A water-soluble crude extracts was obtained from Dendrobium Taiseed Tosnobile by cold water extraction(CWDTT). The data was showed the activities to stimulate macrophages and enhanced the release of NO from RAW264.7 macrophages. Furthermore， the concentration of Interleukin-6(IL-6) and TNF-α were significantly stimulated after exposed to CWDTT。