(a) 重要蘭科花卉品種分子標誌技術開發:。將成功大學、桃園場、臺南場及本場所開發之多組可應用於蝴蝶蘭(包括朵麗蝶蘭)商業品種之SSR引子,以24個共同試驗材料(12個原生種及12個商業品種)進行鑑別能力的篩選與測試。分析結果顯示有14組引子對具有較高的多型性與鑑別能力。並進一步以相似度高的20組品種權品種與對照品種進一步測試後,發現僅有大白花的旭東威士忌與V3無法鑑別,其他各組登記品種與對照品種,在單組或多組SSR分子標誌組合後,皆能被鑑別。未來將進一步由此14組SSR引子對中,挑選更佳之引子組合,並建立此分子鑑定系統的參考品種,並將試驗方法撰寫為成熟的標準試驗方法。(a-1)委託成功大學生物科學系吳副教授文鑾針對鑑別蝴蝶蘭(Phalaenopsis spp.)品種分子標誌技術進行整合。本年度利用一套共同的試驗材料,透過雙方共同測試比較SSR分子標誌之鑑別力,選出其中十組高可擴增率且高度鑑別力的SSR分子標誌,其PIC (polymorphism information content)值介於0.877 (NCKU4)至0.941 (NCKU3)之間。另外,分析十組SSR分子標誌出現頻率最高的61個對偶基因,並選定可涵蓋這些對偶基因的十個蝴蝶蘭品種,作為未來基因型鑑定流程的參考品種,以確認每次鑑定流程的結果可重複性。。(b) 檢定人員訓練及植物品種權教育推廣:。已於2012年10月31日舉辨「2012年作物新品種檢定講習會」,參與人數共計101人。本次研習會講題除介紹一些新作物檢定方法外,亦介紹「蝴蝶蘭品種影像辨識系統」及「數位影像編輯軟體GIMP」之使用方式,為應用於檢定作業及性狀表開發的輔助工具。另外,更以「蝴蝶蘭品種權之國際合作」一題,詳細的介紹並說明臺灣的品種權將以蝴蝶蘭為首開展國際合作,提供有興趣的業者相關資訊,也促進檢定人員了解國際合作的概念及發展方式。。(c) 木瓜三倍體選育運用於品種保護研究:。以上年度利用秋水仙素1.5%處理幼苗莖頂,以流式細胞儀測定認定為4倍體植株後,定植於田間,進行自交及雜交,其自交一代及雜交一代經流式細胞儀檢測獲得10株4倍體植株,再經壓片鏡檢只有1株確認為4倍體。。
英文摘要
The projects of this program have three components. One of the components is to establish varieties identification through SSR molecular marker of new and/or commercial varieties of Phalaenopsis. Ten out of 33 polymorphic SSR markers developed from NCKU(National Cheng Kung University), TDARE(Taoyuan District Agricultural Research and Extension Station), TDARE(Tainan District Agricultural Research and Extension Station) and TSS (Taiwan Seed Improvement and Propagation Station) were selected through testing the transferability and discriminative power among 12 Phalaenopsis species and 12 Phalaenopsis cultivars, and the PIC(polymorphism information content) ranged from 0.69 (Pm4077) to 0.94(NCKU2, NCKU3, NCKU9). The results showed that these 14 SSR markers can be used to complement the DUS test for identifying varieties with similar morphology effectively. 。The second is to training the faculty members to know the relative technologies of DUS test and system of plant variety protection through technique short-term training and keynote speech.The other one is tripolid papaya selection and improvement. Last year use of colchicines over 1.5% top of seedling stems, by flow cytometry after the determination deemed to be tetrapolid of plant, planting in the fields, selfing and hybridization, self-generation and their hybrids through flow cytometry detection. There were 10 plants multipolid and then confirmed by microscopy only 1 for tetrapolid.。The results of this program will provide helpfully informations of plant variety protection.。
(a) 重要蘭科花卉品種分子標誌技術開發:。將成功大學、桃園場、臺南場及本場所開發之多組可應用於蝴蝶蘭(包括朵麗蝶蘭)商業品種之SSR引子,以24個共同試驗材料(12個原生種及12個商業品種)進行鑑別能力的篩選與測試。分析結果顯示有14組引子對具有較高的多型性與鑑別能力。並進一步以相似度高的20組品種權品種與對照品種進一步測試後,發現僅有大白花的旭東威士忌與V3無法鑑別,其他各組登記品種與對照品種,在單組或多組SSR分子標誌組合後,皆能被鑑別。未來將進一步由此14組SSR引子對中,挑選更佳之引子組合,並建立此分子鑑定系統的參考品種,並將試驗方法撰寫為成熟的標準試驗方法。(a-1)委託成功大學生物科學系吳副教授文鑾針對鑑別蝴蝶蘭(Phalaenopsis spp.)品種分子標誌技術進行整合。本年度利用一套共同的試驗材料,透過雙方共同測試比較SSR分子標誌之鑑別力,選出其中十組高可擴增率且高度鑑別力的SSR分子標誌,其PIC (polymorphism information content)值介於0.877 (NCKU4)至0.941 (NCKU3)之間。另外,分析十組SSR分子標誌出現頻率最高的61個對偶基因,並選定可涵蓋這些對偶基因的十個蝴蝶蘭品種,作為未來基因型鑑定流程的參考品種,以確認每次鑑定流程的結果可重複性。。(b) 檢定人員訓練及植物品種權教育推廣:。已於2012年10月31日舉辨「2012年作物新品種檢定講習會」,參與人數共計101人。本次研習會講題除介紹一些新作物檢定方法外,亦介紹「蝴蝶蘭品種影像辨識系統」及「數位影像編輯軟體GIMP」之使用方式,為應用於檢定作業及性狀表開發的輔助工具。另外,更以「蝴蝶蘭品種權之國際合作」一題,詳細的介紹並說明臺灣的品種權將以蝴蝶蘭為首開展國際合作,提供有興趣的業者相關資訊,也促進檢定人員了解國際合作的概念及發展方式。。(c) 木瓜三倍體選育運用於品種保護研究:。以上年度利用秋水仙素1.5%處理幼苗莖頂,以流式細胞儀測定認定為4倍體植株後,定植於田間,進行自交及雜交,其自交一代及雜交一代經流式細胞儀檢測獲得10株4倍體植株,再經壓片鏡檢只有1株確認為4倍體。。
英文摘要
The projects of this program have three components. One of the components is to establish varieties identification through SSR molecular marker of new and/or commercial varieties of Phalaenopsis. Ten out of 33 polymorphic SSR markers developed from NCKU(National Cheng Kung University), TDARE(Taoyuan District Agricultural Research and Extension Station), TDARE(Tainan District Agricultural Research and Extension Station) and TSS (Taiwan Seed Improvement and Propagation Station) were selected through testing the transferability and discriminative power among 12 Phalaenopsis species and 12 Phalaenopsis cultivars, and the PIC(polymorphism information content) ranged from 0.69 (Pm4077) to 0.94(NCKU2, NCKU3, NCKU9). The results showed that these 14 SSR markers can be used to complement the DUS test for identifying varieties with similar morphology effectively. 。The second is to training the faculty members to know the relative technologies of DUS test and system of plant variety protection through technique short-term training and keynote speech.The other one is tripolid papaya selection and improvement. Last year use of colchicines over 1.5% top of seedling stems, by flow cytometry after the determination deemed to be tetrapolid of plant, planting in the fields, selfing and hybridization, self-generation and their hybrids through flow cytometry detection. There were 10 plants multipolid and then confirmed by microscopy only 1 for tetrapolid.。The results of this program will provide helpfully informations of plant variety protection.。