本計畫結果如下。1.在培養室中建立一套10種不同型式(T5- fluorescent lamps)之光線控制系統。。2.利用3種不同不週期調查對2種彩色海芋(cv. Florex and Pacific pink)及一種石斛蘭對芽體增殖之影響。彩色海芋之芽體置於3種不同光週期(8、12、16-h光期)，30天後，2種彩色海芋在12及16-h光期下培養之芽體高度高於8-h光期，Florex and Pacific pink芽體增殖倍率在12及16-h光期下培養明顯高於8-h光期。芽體鮮重與乾重在8-h光期下明顯降低，在12-h及16-h二種光週期比較下在芽體高度、芽體增殖倍率、鮮重及乾重均不影響。在石斛蘭(cv. Taiseed Tosnobile)上，在8-h及12-h二種光週期下培養芽體高度、芽體增殖倍率、鮮重及乾重均會明顯比在16-h光期下降低。。3.在2種彩色海芋發根培養於塑膠培養瓶中以單層或雙層封膜試驗中，雙層封膜可顯著降低瓶苗之污染，而且雙層封膜不影響芽體高度、發根的數量及芽體鮮乾重。。
英文摘要
The results of this program are examined see below:。1. Set one auto-control system of 10 different lights (T5- fluorescent lamps) for tissue culture in culture room.。2. The effects of 3 different photoperiods on shoot proliferation from 2 calla lily cultivars (cv. Florex and Pacific pink) and 1 dendrobium micro-shoots were tested. Shoot cluster of calla lily were cultured in culture room under 8， 12， and 16-h photoperiods. After 30 d， The shoots of 2 calla lily cultivars cultured under the 12 and 16-h photoperiods were taller than those grown under 8 h. The shoot proliferation rate of Florex and Pacific pink increased when plants were grown at 12 and 16-h photoperiods. Reduction in fresh and dry weights of shoots was significant already under 8-h photoperiods. 12 and 16-h photoperiods had no significant in shoot height， shoot proliferation rate， fresh and dry weights. In dendrobium (cv. Taiseed Tosnobile) Reduction in shoot height，shoot proliferation rate， fresh and dry weights of shoots was significant under 8-h and 12-h photoperiods.。3. In this experiment of sealing with membrane for tissue culture. 2 calla lily cultivars (cv. Florex and Pacific pink) rooting culture by plastic vessels and sealing with one or two layer membrane. Reduction contamination was significant by plastic vessels sealing with two layer membrane. Furthermore， sealing with two layer membranes had no significant in shoot height， the number of rooting， fresh and dry weights.。
本計畫結果如下。1.在培養室中建立一套10種不同型式(T5- fluorescent lamps)之光線控制系統。。2.利用3種不同不週期調查對2種彩色海芋(cv. Florex and Pacific pink)及一種石斛蘭對芽體增殖之影響。彩色海芋之芽體置於3種不同光週期(8、12、16-h光期)，30天後，2種彩色海芋在12及16-h光期下培養之芽體高度高於8-h光期，Florex and Pacific pink芽體增殖倍率在12及16-h光期下培養明顯高於8-h光期。芽體鮮重與乾重在8-h光期下明顯降低，在12-h及16-h二種光週期比較下在芽體高度、芽體增殖倍率、鮮重及乾重均不影響。在石斛蘭(cv. Taiseed Tosnobile)上，在8-h及12-h二種光週期下培養芽體高度、芽體增殖倍率、鮮重及乾重均會明顯比在16-h光期下降低。。3.在2種彩色海芋發根培養於塑膠培養瓶中以單層或雙層封膜試驗中，雙層封膜可顯著降低瓶苗之污染，而且雙層封膜不影響芽體高度、發根的數量及芽體鮮乾重。。
英文摘要
The results of this program are examined see below:。1. Set one auto-control system of 10 different lights (T5- fluorescent lamps) for tissue culture in culture room.。2. The effects of 3 different photoperiods on shoot proliferation from 2 calla lily cultivars (cv. Florex and Pacific pink) and 1 dendrobium micro-shoots were tested. Shoot cluster of calla lily were cultured in culture room under 8， 12， and 16-h photoperiods. After 30 d， The shoots of 2 calla lily cultivars cultured under the 12 and 16-h photoperiods were taller than those grown under 8 h. The shoot proliferation rate of Florex and Pacific pink increased when plants were grown at 12 and 16-h photoperiods. Reduction in fresh and dry weights of shoots was significant already under 8-h photoperiods. 12 and 16-h photoperiods had no significant in shoot height， shoot proliferation rate， fresh and dry weights. In dendrobium (cv. Taiseed Tosnobile) Reduction in shoot height，shoot proliferation rate， fresh and dry weights of shoots was significant under 8-h and 12-h photoperiods.。3. In this experiment of sealing with membrane for tissue culture. 2 calla lily cultivars (cv. Florex and Pacific pink) rooting culture by plastic vessels and sealing with one or two layer membrane. Reduction contamination was significant by plastic vessels sealing with two layer membrane. Furthermore， sealing with two layer membranes had no significant in shoot height， the number of rooting， fresh and dry weights.。