The projects of this program have three components including.The first is the establishment and application molecular markers of the resistance to sport wilt virus in tomato analysis system. An allelicspecific markers SCAR-Sw 5-1313 and SCAR-sw 5-676 were developed for differentiating allelic variation at Sw5 locus. It amplifies fragments。from both resistant and susceptible tomato accessions with different molecular sizes that corresponding to the presence of Sw5/Sw5， Sw5/sw5 and sw5/sw5 alleles. Sw5a and Sw5b were resistance gene to sport wilt virus in chromosome 9 of tomato. Genes (Sw 5a and Sw 5b) could be identified by molecular markers Tss#SW5a and Tss#SW5b， respectively. F2 segreation rate of Sw5/Sw5， Sw5/sw5 and sw5/sw5 was 1:2:1.The second is to establishment the molecular markers for sex determination in Carica papaya (papaya) by using RAPDand SCAR analysis.Marker Napf76/77-800 was specific to hermaphrodite and maleness. Marker。CFw/CRv-700 was specific to female.Marker Ypa7F/Ypa7R-1000 was specific to maleness. The markers could be applied for sexual identification in papaya seedling stage.The third is studing in multi-genes of agricultural anthracanose disease resistance in Capsicum spp by proteiomic analysis using twodimention electrophoresis. We utilized 2-D electrophoresis to screen the variational point of proteins between anthracnose- resistant and susceptible line extracted from the leaves and fruits of hot pepper.Partial point sequencing has been accomplished and we are currently undergoing protein type analysis and sequence conversions to establish the molecular markers differentiating anthrancnose-resistant lines.。
The projects of this program have three components including.The first is the establishment and application molecular markers of the resistance to sport wilt virus in tomato analysis system. An allelicspecific markers SCAR-Sw 5-1313 and SCAR-sw 5-676 were developed for differentiating allelic variation at Sw5 locus. It amplifies fragments。from both resistant and susceptible tomato accessions with different molecular sizes that corresponding to the presence of Sw5/Sw5， Sw5/sw5 and sw5/sw5 alleles. Sw5a and Sw5b were resistance gene to sport wilt virus in chromosome 9 of tomato. Genes (Sw 5a and Sw 5b) could be identified by molecular markers Tss#SW5a and Tss#SW5b， respectively. F2 segreation rate of Sw5/Sw5， Sw5/sw5 and sw5/sw5 was 1:2:1.The second is to establishment the molecular markers for sex determination in Carica papaya (papaya) by using RAPDand SCAR analysis.Marker Napf76/77-800 was specific to hermaphrodite and maleness. Marker。CFw/CRv-700 was specific to female.Marker Ypa7F/Ypa7R-1000 was specific to maleness. The markers could be applied for sexual identification in papaya seedling stage.The third is studing in multi-genes of agricultural anthracanose disease resistance in Capsicum spp by proteiomic analysis using twodimention electrophoresis. We utilized 2-D electrophoresis to screen the variational point of proteins between anthracnose- resistant and susceptible line extracted from the leaves and fruits of hot pepper.Partial point sequencing has been accomplished and we are currently undergoing protein type analysis and sequence conversions to establish the molecular markers differentiating anthrancnose-resistant lines.。