基因轉殖作物檢監測體系由農糧署委託種苗改良繁殖場邀集農業試驗所、桃園區農業改良場、台南區農業改良場、花蓮區農業改良場、國立中興大學等單位建立「基因轉殖作物檢測監測小組」,藉由「檢測技術建立」、「儀器與檢測精準度試驗」、「檢測樣品檢出能力試驗」、「能力試驗」等,已建立抗輪點病毒病木瓜、雙重抗木瓜輪點病毒及木瓜畸葉嵌紋病毒性狀基因轉殖木瓜、衛生署公告許可之上市品項之基因轉殖玉米及大豆等建立標準化檢監測模式,並利用multiplex PCR技術進行基因轉殖木瓜檢測,可在1次PCR反應中同時檢測papain gene、nptII gene、py16-cp gene與prsv-cp gene。其中基因轉殖木瓜及大豆種苗場已依據國際品保標準I S O / I E C 17025取得「財團法人全國認證基金會(Taiwan Accreditation Foundation, TAF)」之生物領域測試實驗室認證,建立基因轉殖作物種苗認驗證體系。另蒐集國內外核酸定量檢測之量測不確定度(measurement uncertainty, MU)、國內外基因轉殖玉米與大豆花粉飄散與傳統農業共存(coexistence)栽培模式研究成果,建立基因轉殖作物標準化取樣及監測模式並以田間試驗模擬基因轉殖玉米飄散趨勢,建立預測模式。亦配合種苗改良繁殖場利用衛星定位(GPS)調查台灣玉米、大豆栽培區資料,以地理資訊系統(Geographic Information System, GIS)分析是否受到污染,並完成 GIS 規劃報告及 舉辦「基因轉殖作物生物安全座談會」。
英文摘要
The detecting and monitoring system of transgenic crops in Taiwan was entrusted by the Council of Agriculture which invited Agricultural Research institute, Taoyuan District Agricultural Research and Extension Station, Tainan District Agricultural Research and Extension Station,Hualien District Agricultural Research and Extension Station, National。Chung Hsing University and Taiwan Seed Improvement and Propagation Station to form the team of detecting and monitoring transgenic crops. By means of accuracy, detection capability and ability test, the standard detecting and monitoring model have already been built up. In addition,the technique of multiple PCR was employed to detect transgenic papaya.The papain gene,nptII gene , py16-cp gene could be detected simultaneously in one PCR reaction. The biological area of detecting lab of SIPS has been accredited for transgenic papaya and soybean by TAF based on ISO/IEC 17025. In this study, the literatures of measurement uncertainty (MU), the dispersal of pollen flow for maize and soybean, and。the coexistence between conventional and transgenic maize and soybean have been collected and reviewed. The standard sampling and monitoring model was built up and a simulated study of dispersal of transgenic maize in the field was also processed in TARI to investigate dispersal tendency. The survey of maize and soybean production farms in Taiwan was executed by GPS and the technique of GIS was employed to analyze whether those production farms were polluted by transgenic species. A proposal for the GIS was finalized and a conference of biosafety for transgenic crops was hosted by SIPS.。
基因轉殖作物檢監測體系由農糧署委託種苗改良繁殖場邀集農業試驗所、桃園區農業改良場、台南區農業改良場、花蓮區農業改良場、國立中興大學等單位建立「基因轉殖作物檢測監測小組」,藉由「檢測技術建立」、「儀器與檢測精準度試驗」、「檢測樣品檢出能力試驗」、「能力試驗」等,已建立抗輪點病毒病木瓜、雙重抗木瓜輪點病毒及木瓜畸葉嵌紋病毒性狀基因轉殖木瓜、衛生署公告許可之上市品項之基因轉殖玉米及大豆等建立標準化檢監測模式,並利用multiplex PCR技術進行基因轉殖木瓜檢測,可在1次PCR反應中同時檢測papain gene、nptII gene、py16-cp gene與prsv-cp gene。其中基因轉殖木瓜及大豆種苗場已依據國際品保標準I S O / I E C 17025取得「財團法人全國認證基金會(Taiwan Accreditation Foundation, TAF)」之生物領域測試實驗室認證,建立基因轉殖作物種苗認驗證體系。另蒐集國內外核酸定量檢測之量測不確定度(measurement uncertainty, MU)、國內外基因轉殖玉米與大豆花粉飄散與傳統農業共存(coexistence)栽培模式研究成果,建立基因轉殖作物標準化取樣及監測模式並以田間試驗模擬基因轉殖玉米飄散趨勢,建立預測模式。亦配合種苗改良繁殖場利用衛星定位(GPS)調查台灣玉米、大豆栽培區資料,以地理資訊系統(Geographic Information System, GIS)分析是否受到污染,並完成 GIS 規劃報告及 舉辦「基因轉殖作物生物安全座談會」。
英文摘要
The detecting and monitoring system of transgenic crops in Taiwan was entrusted by the Council of Agriculture which invited Agricultural Research institute, Taoyuan District Agricultural Research and Extension Station, Tainan District Agricultural Research and Extension Station,Hualien District Agricultural Research and Extension Station, National。Chung Hsing University and Taiwan Seed Improvement and Propagation Station to form the team of detecting and monitoring transgenic crops. By means of accuracy, detection capability and ability test, the standard detecting and monitoring model have already been built up. In addition,the technique of multiple PCR was employed to detect transgenic papaya.The papain gene,nptII gene , py16-cp gene could be detected simultaneously in one PCR reaction. The biological area of detecting lab of SIPS has been accredited for transgenic papaya and soybean by TAF based on ISO/IEC 17025. In this study, the literatures of measurement uncertainty (MU), the dispersal of pollen flow for maize and soybean, and。the coexistence between conventional and transgenic maize and soybean have been collected and reviewed. The standard sampling and monitoring model was built up and a simulated study of dispersal of transgenic maize in the field was also processed in TARI to investigate dispersal tendency. The survey of maize and soybean production farms in Taiwan was executed by GPS and the technique of GIS was employed to analyze whether those production farms were polluted by transgenic species. A proposal for the GIS was finalized and a conference of biosafety for transgenic crops was hosted by SIPS.。