為協助業者將優質植物種苗（子）行銷國外市場，提升農產業競爭力，本研究規劃一完整且即時能提供各國植物種苗（子）檢疫資料庫，蒐集相關法規，共計836條條文，期能縮短資料檢索時間。。文獻搜尋並比較國際試驗室間檢測番茄嵌紋病毒(Tomato Mosaic Virus，ToMV)的檢測方式保括ELISA、RT-PCR、ICRT-PCR等檢測技術，本計畫前期合成針對ToMV專一之引子對以及植體之Internal control primer set， 可建立RT-PCR偵測受檢檢體內之ToMV，確定檢測之正確率。。研究發現H2O2及乾熱處理對黑腐病污染及田間收取之種子皆有良好的減菌效果，藉由處理條件之最佳化調整，可有效殺滅表面攜帶之菌類。苦瓜種子發芽最適合之處理為60℃溫湯浸種10分鐘，發芽率可達80 %以上，沙床法與捲紙法可提高發芽率至90%以上。。建立胜肽親和性篩選系統(peptides c7c， peptides 7， peptides 12)對於標地蛋白之親和性汰選技術，建立及生產與ORSV病毒鞘蛋白具親和性之噬菌體，篩選出可利用於ELIS檢測ORSV之噬菌體。。
英文摘要
For the purposes to help entrepreneurs marketing high grade crop seeds and seedlings overseas， and upgrading competitive power of agricultural industries， we have mapped out a complete database collecting 836 articles in total which provides various countries’ quarantine regulations immediately. Hopefully， this can reduce time for data searching.。By papers literature research， we selected the RT-PCR as the major tech as our TAF testing method for Tomato Mosaic Virus. At the first we design a set of RT-PCR primer for ToMV specific primer set. We also design the internal control primer set for more reliable detection result.。The protocol of H2O2 treatment followed by heating dry for killing Xanthomonas campestris pv. campestris (XCC) on cabbage seeds were evaluated to show better effects. A more précised H2O2 treatment will also be a pursuit.。Bitter gourd seeds immersed in ℃ water for 10 min， the germination rate could be reached above 80 %. The seeds at the sand bad or the paper roll were increased the germination rate above 90 %.By phage display system，there were three kinds of phage library being selected by affinity panning selection including phage c7c library， phage 7 library and phage 12 library. In phage c7c system， there were five phage populations being selected to do the indirect ELISA test for ORSV coat protein. By the ELISA test result， the specific peptides regions of these five phage populations were sequencing.。
為協助業者將優質植物種苗（子）行銷國外市場，提升農產業競爭力，本研究規劃一完整且即時能提供各國植物種苗（子）檢疫資料庫，蒐集相關法規，共計836條條文，期能縮短資料檢索時間。。文獻搜尋並比較國際試驗室間檢測番茄嵌紋病毒(Tomato Mosaic Virus，ToMV)的檢測方式保括ELISA、RT-PCR、ICRT-PCR等檢測技術，本計畫前期合成針對ToMV專一之引子對以及植體之Internal control primer set， 可建立RT-PCR偵測受檢檢體內之ToMV，確定檢測之正確率。。研究發現H2O2及乾熱處理對黑腐病污染及田間收取之種子皆有良好的減菌效果，藉由處理條件之最佳化調整，可有效殺滅表面攜帶之菌類。苦瓜種子發芽最適合之處理為60℃溫湯浸種10分鐘，發芽率可達80 %以上，沙床法與捲紙法可提高發芽率至90%以上。。建立胜肽親和性篩選系統(peptides c7c， peptides 7， peptides 12)對於標地蛋白之親和性汰選技術，建立及生產與ORSV病毒鞘蛋白具親和性之噬菌體，篩選出可利用於ELIS檢測ORSV之噬菌體。。
英文摘要
For the purposes to help entrepreneurs marketing high grade crop seeds and seedlings overseas， and upgrading competitive power of agricultural industries， we have mapped out a complete database collecting 836 articles in total which provides various countries’ quarantine regulations immediately. Hopefully， this can reduce time for data searching.。By papers literature research， we selected the RT-PCR as the major tech as our TAF testing method for Tomato Mosaic Virus. At the first we design a set of RT-PCR primer for ToMV specific primer set. We also design the internal control primer set for more reliable detection result.。The protocol of H2O2 treatment followed by heating dry for killing Xanthomonas campestris pv. campestris (XCC) on cabbage seeds were evaluated to show better effects. A more précised H2O2 treatment will also be a pursuit.。Bitter gourd seeds immersed in ℃ water for 10 min， the germination rate could be reached above 80 %. The seeds at the sand bad or the paper roll were increased the germination rate above 90 %.By phage display system，there were three kinds of phage library being selected by affinity panning selection including phage c7c library， phage 7 library and phage 12 library. In phage c7c system， there were five phage populations being selected to do the indirect ELISA test for ORSV coat protein. By the ELISA test result， the specific peptides regions of these five phage populations were sequencing.。