為獲得穩定的仙履蘭炭疽病菌(C. gloeosporioides)的接種源，已合成一個能有效促進產胞的培養基。針對仙履蘭炭疽病菌對蘭科或非蘭科寄主的專一性，得知該菌可為害蝴蝶蘭、嘉得利亞蘭、國蘭及文心蘭等蘭科植物，但不會為害非蘭科的作物。評估仙履蘭四品種對炭疽病的抗感性，結果顯示四品種均可被此病原感染，其中以iveum x sib和Hung Sheng Glow x Incantation雜交後裔最為感病。測試七種精油對仙履蘭炭疽病菌生長之影響，結果顯示草果與冬青精油抑菌的效果最佳。網室評估三種不同精油配方防治炭疽病菌的效果，結果顯示施用AF1-99配方配合撲克拉錳(6000倍)的防治的效果比單獨施用AF1-99配方或撲克拉錳的防治效果較佳。網室評估四支木黴菌防治炭疽病菌的效果，結果顯示T. viride與 T. harzianum兩支木黴菌可有效降低炭疽病的發生率。為達到早期病害檢測之目標，本試驗針對仙履蘭炭疽病菌設計專一性引子對90F/328R：(3'-GAC TTT GAA ATG CGA CTA CAG-5')/ (3'- GCC TTG CGA CGG AAC ATG GCG-5')可專一性偵測仙履蘭炭疽病菌，於300 bp可擴增一特異性條帶。進一步利用孢子懸浮液、菌株以及健康植株與菌株DNA不同混合比例，測試此專一性引子對之靈敏度。結果指出，於孢子濃度約100顆孢子/μl，或菌株DNA濃度約1 ng/μl，或植菌比為50：1，皆可有效檢測出仙履蘭炭疽病菌。
英文摘要
In order to obtain a stable inocula of Colletotrichum gloeosporioides， a medium was formulated for enhancing sporulation of C. gloeosporioides. Orchid or nonorchid plants were inoculated to etermine host specificity of C. gloeosporioides. C.gloeosporioides was mainly pathogenic on Phalaenopsis， Cattleya， Cymbidium and Oncidium orchids. Four cultivars of Paphiopedilum were evaluated for their response to C . gloeosporioides. Among four cultivars tested， two hybrid descendants niveum x siband and Hung Sheng Glow x Incantation were the most susceptible. Seven essential oils were investigated for their antifungal activities on C. gloeosporioides. Essential oils obtained from Amomum tsao-ko and Ilex were the most effective for suppressing mycelial growth of C. gloeosporioides. Three essential oil formulations were studied their antifungal activities on C.gloeosporioides in greenhouse. Results showed combination of AF1-99 oil formula and prochloraz manganese (6000 times) was the most effective for controlling the incidence of anthracnose disease， compared with AF1-99 formula alone or prochloraz manganese (6000 times) alone. In greenhouse experiment， four isolates of Trichoderma were tested for their abilities for control of anthracnose.Among four Trichoderma isolates tested， T. viride and T. harzianum effectively reduced the incidence of anthracnose disease. A set of specific primers， 90F/328R:(3’-GAC TTT GAA ATG CGA CTA CAG-5’)/ (3’- GCC TTG CGA CGG AAC ATG GCG -5’)， was desiged to amplify a portion of the β-tubulin gene for the detection of C. gloeosporioides from slipper orchid. Results showed that a DNA fragment of about 300 bp was only amplified in slipper orchid， not in C.gloeosporioides from other crops. The primers sensitivity were assessed further by using different spore suspension(10000， 2000， 1000， 200， 100， 50， 1 spores/l)， different fungal DNA(10， 1， 0.1， 0.01， 0.001 ng/l)， and ratio of plant DNA: fungal DNA(0：1、5：1、10：1、50：1、100：1、150：1、200：1、250：1、300：1 、1：0). The sensitivity using the specific primers was 100 spores/l suspension，1 ng/l DNA or ratio of 50:1(plant DNA: fungal DNA)， respectively.
為獲得穩定的仙履蘭炭疽病菌(C. gloeosporioides)的接種源，已合成一個能有效促進產胞的培養基。針對仙履蘭炭疽病菌對蘭科或非蘭科寄主的專一性，得知該菌可為害蝴蝶蘭、嘉得利亞蘭、國蘭及文心蘭等蘭科植物，但不會為害非蘭科的作物。評估仙履蘭四品種對炭疽病的抗感性，結果顯示四品種均可被此病原感染，其中以iveum x sib和Hung Sheng Glow x Incantation雜交後裔最為感病。測試七種精油對仙履蘭炭疽病菌生長之影響，結果顯示草果與冬青精油抑菌的效果最佳。網室評估三種不同精油配方防治炭疽病菌的效果，結果顯示施用AF1-99配方配合撲克拉錳(6000倍)的防治的效果比單獨施用AF1-99配方或撲克拉錳的防治效果較佳。網室評估四支木黴菌防治炭疽病菌的效果，結果顯示T. viride與 T. harzianum兩支木黴菌可有效降低炭疽病的發生率。為達到早期病害檢測之目標，本試驗針對仙履蘭炭疽病菌設計專一性引子對90F/328R：(3'-GAC TTT GAA ATG CGA CTA CAG-5')/ (3'- GCC TTG CGA CGG AAC ATG GCG-5')可專一性偵測仙履蘭炭疽病菌，於300 bp可擴增一特異性條帶。進一步利用孢子懸浮液、菌株以及健康植株與菌株DNA不同混合比例，測試此專一性引子對之靈敏度。結果指出，於孢子濃度約100顆孢子/μl，或菌株DNA濃度約1 ng/μl，或植菌比為50：1，皆可有效檢測出仙履蘭炭疽病菌。
英文摘要
In order to obtain a stable inocula of Colletotrichum gloeosporioides， a medium was formulated for enhancing sporulation of C. gloeosporioides. Orchid or nonorchid plants were inoculated to etermine host specificity of C. gloeosporioides. C.gloeosporioides was mainly pathogenic on Phalaenopsis， Cattleya， Cymbidium and Oncidium orchids. Four cultivars of Paphiopedilum were evaluated for their response to C . gloeosporioides. Among four cultivars tested， two hybrid descendants niveum x siband and Hung Sheng Glow x Incantation were the most susceptible. Seven essential oils were investigated for their antifungal activities on C. gloeosporioides. Essential oils obtained from Amomum tsao-ko and Ilex were the most effective for suppressing mycelial growth of C. gloeosporioides. Three essential oil formulations were studied their antifungal activities on C.gloeosporioides in greenhouse. Results showed combination of AF1-99 oil formula and prochloraz manganese (6000 times) was the most effective for controlling the incidence of anthracnose disease， compared with AF1-99 formula alone or prochloraz manganese (6000 times) alone. In greenhouse experiment， four isolates of Trichoderma were tested for their abilities for control of anthracnose.Among four Trichoderma isolates tested， T. viride and T. harzianum effectively reduced the incidence of anthracnose disease. A set of specific primers， 90F/328R:(3’-GAC TTT GAA ATG CGA CTA CAG-5’)/ (3’- GCC TTG CGA CGG AAC ATG GCG -5’)， was desiged to amplify a portion of the β-tubulin gene for the detection of C. gloeosporioides from slipper orchid. Results showed that a DNA fragment of about 300 bp was only amplified in slipper orchid， not in C.gloeosporioides from other crops. The primers sensitivity were assessed further by using different spore suspension(10000， 2000， 1000， 200， 100， 50， 1 spores/l)， different fungal DNA(10， 1， 0.1， 0.01， 0.001 ng/l)， and ratio of plant DNA: fungal DNA(0：1、5：1、10：1、50：1、100：1、150：1、200：1、250：1、300：1 、1：0). The sensitivity using the specific primers was 100 spores/l suspension，1 ng/l DNA or ratio of 50:1(plant DNA: fungal DNA)， respectively.