本發明提供一種波導耦合表面電漿共振生物感測器,其係包括一光柵層、一波導層、一電漿共振層以及一配體層。該光柵層係為透光材料且具有週期性之一第一光柵結構,該波導層係形成於該第一光柵結構上,該波導層之折射率係大於該光柵層之折射率。該電漿共振層形成於該波導層上,該電漿共振層可藉由光波激發以產生電漿共振效應。該配體層係形成於該電漿共振層上,該配體層可與待測物質之受體進行結合反應。利用本發明之感測器可建立生物分子間作用分析之平台,以無標記之檢測方式,即時量測生化材料分子間之交互作用、反應速率、分子動力學等物理量,以達成高靈敏度之檢測效能。 The present invention discloses a coupled waveguide-surface plasmon resonance biosensor, comprising: a grating layer formed of a transparent material, the grating layer comprising a first periodic grating structure; a waveguide layer formed on the first periodic grating structure, the refractive index of the waveguide layer being larger than the refractive index of the grating layer; a plasmon resonance layer formed on the waveguide layer, capable of being optically excited to cause a plasmon resonance wave; and a ligand layer formed on the plasmon resonance layer; capable of being bound to react with receptors of a sample to be tested.
技術摘要-中文: 本發明提供一種波導耦合表面電漿共振生物感測器,其係包括一光柵層、一波導層、一電漿共振層以及一配體層。該光柵層係為透光材料且具有週期性之一第一光柵結構,該波導層係形成於該第一光柵結構上,該波導層之折射率係大於該光柵層之折射率。該電漿共振層形成於該波導層上,該電漿共振層可藉由光波激發以產生電漿共振效應。該配體層係形成於該電漿共振層上,該配體層可與待測物質之受體進行結合反應。利用本發明之感測器可建立生物分子間作用分析之平台,以無標記之檢測方式,即時量測生化材料分子間之交互作用、反應速率、分子動力學等物理量,以達成高靈敏度之檢測效能。 The present invention discloses a coupled waveguide-surface plasmon resonance biosensor, comprising: a grating layer formed of a transparent material, the grating layer comprising a first periodic grating structure; a waveguide layer formed on the first periodic grating structure, the refractive index of the waveguide layer being larger than the refractive index of the grating layer; a plasmon resonance layer formed on the waveguide layer, capable of being optically excited to cause a plasmon resonance wave; and a ligand layer formed on the plasmon resonance layer; capable of being bound to react with receptors of a sample to be tested.