a. Development of standardized sampling and testing of the potato disease potato production processes comply with health applications verified.。b.The objective of this program is to develop a convenient and effective seed disinfection technique on asparagus bean for decreasing the ratio of seed carrying Fusarium oxysporum f. sp. tracheiphilum. This disinfection technique will be helpful for diminishing the transmission and occurrence of the Fusarium wilt of asparagus bean in the fields.The Fusarium oxysporum f. sp. tracheiphilum (FOT) carried by asparagus bean seeds could be transmitted by polluting the seed surface or infecting into seeds. The investigation results showed that the FOT was detected on four asparagus bean cultivars with ratio of 3-25%. Other fungi and bacteria were also detected with high seed-carried ratio， but，after surface disinfection， the ratio of some fungi and bacteria decreased effectively. However， it was still possible that the fungi and bacteria existed in seeds. The result of disinfection with fungicides indicated that the effect of chemicals were not able to be confirmed yet owing to FOT-carried ratio on asparagus seeds were low and not stable.。c.c-1 Strawberry disease non-pesticide control technology development :research for prevention and treatment of strawberry anthracnose nonpesticide formulations to reduce the amount of chemical pesticides，reducing pesticide residues on human health and the environment and ecology of the risk of harm. (1)Application of different concentrations of chitin in strawberry tissue culture medium. Processing plants 100 ppm concentration of chitin its anthrax lesion smaller and the processing of chitin concentrations no other impact.(2) Strawberry seedlings treated with different concentrations of chitin treatment after picking leaves and do not pick the leaves inoculated with anthrax bacteria. Strawberry seedlings treated with different concentrations of chitin， its initial investigation of anthrax lower disease severity compared to the control .The results showed that treatment plants do not pick off the leaves and leaf compared， the results do not pick leaves treatment had lower mortality. c-2 26 heat-resistant bacteria were isolated from field soil collected in central， southern and eastern Taiwan. By dual culture， five of them showed ability to inhibit the strawberry anthracnose mycelia growth on PDA by more than 44.62% have been selected as antagonists. The 24-hr cell cultures of these 5 antagonists could inhibit germing of pathogen spores or elongating of the germ tubes. While biocontrol trials，cell cultures of t he antagonists were spreaded on strawberry leaves before wounded and inoculated with anthracnose. 1 week later， antagonists No. B2014-12 and B2014-21 made the lesions slightly smaller CK treated.No. B2014-12 could also decrease 16% lesion area when treated after wounding and inoculating. Continuous treatment with antagonists cell culture 3days before inoculating by spray conidia suspension， No. B2014-12 could decrease 2.3-4% disease severity on strawberry than CK. Among commercial biopesticides， Bs was most effective on controlling this disease decrease 4.3-12.5% disease severity on plants.。d.Antiviral serum preparation technology development and use:Before enter a large number of plant tissue culture propagation， to detect the plant target disease can ensure a healthy production procedure. The specific pathogen free tissue culture units also are the cornerstone for the production of healthy seedlings. Tissue culture is the subject diseases including viral diseases， viral diseases and class mycoplasma disease. And techniques for detect these pathogen are enzymelinked immuno-sorbent assay， ELISA and Reverse transcription-PCR， RT-PCR.The cost of serum is the highest value of ELISA. This project is intended to express recombinant proteins in transgenic E. coli， the subject of the virus as a source of protein production， the production of important crops of the serum， the plans to complete the production of Grape virus A， GVA of the production each 80ml， diluted potency up to 8000-16，000 times.。
a. Development of standardized sampling and testing of the potato disease potato production processes comply with health applications verified.。b.The objective of this program is to develop a convenient and effective seed disinfection technique on asparagus bean for decreasing the ratio of seed carrying Fusarium oxysporum f. sp. tracheiphilum. This disinfection technique will be helpful for diminishing the transmission and occurrence of the Fusarium wilt of asparagus bean in the fields.The Fusarium oxysporum f. sp. tracheiphilum (FOT) carried by asparagus bean seeds could be transmitted by polluting the seed surface or infecting into seeds. The investigation results showed that the FOT was detected on four asparagus bean cultivars with ratio of 3-25%. Other fungi and bacteria were also detected with high seed-carried ratio， but，after surface disinfection， the ratio of some fungi and bacteria decreased effectively. However， it was still possible that the fungi and bacteria existed in seeds. The result of disinfection with fungicides indicated that the effect of chemicals were not able to be confirmed yet owing to FOT-carried ratio on asparagus seeds were low and not stable.。c.c-1 Strawberry disease non-pesticide control technology development :research for prevention and treatment of strawberry anthracnose nonpesticide formulations to reduce the amount of chemical pesticides，reducing pesticide residues on human health and the environment and ecology of the risk of harm. (1)Application of different concentrations of chitin in strawberry tissue culture medium. Processing plants 100 ppm concentration of chitin its anthrax lesion smaller and the processing of chitin concentrations no other impact.(2) Strawberry seedlings treated with different concentrations of chitin treatment after picking leaves and do not pick the leaves inoculated with anthrax bacteria. Strawberry seedlings treated with different concentrations of chitin， its initial investigation of anthrax lower disease severity compared to the control .The results showed that treatment plants do not pick off the leaves and leaf compared， the results do not pick leaves treatment had lower mortality. c-2 26 heat-resistant bacteria were isolated from field soil collected in central， southern and eastern Taiwan. By dual culture， five of them showed ability to inhibit the strawberry anthracnose mycelia growth on PDA by more than 44.62% have been selected as antagonists. The 24-hr cell cultures of these 5 antagonists could inhibit germing of pathogen spores or elongating of the germ tubes. While biocontrol trials，cell cultures of t he antagonists were spreaded on strawberry leaves before wounded and inoculated with anthracnose. 1 week later， antagonists No. B2014-12 and B2014-21 made the lesions slightly smaller CK treated.No. B2014-12 could also decrease 16% lesion area when treated after wounding and inoculating. Continuous treatment with antagonists cell culture 3days before inoculating by spray conidia suspension， No. B2014-12 could decrease 2.3-4% disease severity on strawberry than CK. Among commercial biopesticides， Bs was most effective on controlling this disease decrease 4.3-12.5% disease severity on plants.。d.Antiviral serum preparation technology development and use:Before enter a large number of plant tissue culture propagation， to detect the plant target disease can ensure a healthy production procedure. The specific pathogen free tissue culture units also are the cornerstone for the production of healthy seedlings. Tissue culture is the subject diseases including viral diseases， viral diseases and class mycoplasma disease. And techniques for detect these pathogen are enzymelinked immuno-sorbent assay， ELISA and Reverse transcription-PCR， RT-PCR.The cost of serum is the highest value of ELISA. This project is intended to express recombinant proteins in transgenic E. coli， the subject of the virus as a source of protein production， the production of important crops of the serum， the plans to complete the production of Grape virus A， GVA of the production each 80ml， diluted potency up to 8000-16，000 times.。