The seeds of Pachira macrocarpa were collected， and treated with 0mM，0.5mM， 1mM and 2mM for 24hrs or 48hrs. After treatments， the hygrochastic seeds were planted apart. Then counted the germination rates of seeds and observed if there was mutation. The germination rate of hygrachastic seeds treated with 0， 0.5， 1， 2mM NaN3 for 24hrs were 1.43%， 10%，22.86% and 11.76%. After 48hr， the germination rate were 0%， 0%， 1.52%，12.12%. There have been three mutated plants induced by NaN3 treatment.One is with leaf variegation along the central vines and the others are with thinner and undulated leaves. As to callus induction， medium with 12，4-D 1mg/L had the best effect on callus induction， and 2，4-D 1mg/L caused browning frequently. Under the other treatments， explants grew greenly and had less calli. Most of calli formed from the cut places of explants， and were friable without embro-like body. Calli subcultured in medium with 2，4-D and 2ip can keep growing， but still without embryo-like body.
The seeds of Pachira macrocarpa were collected， and treated with 0mM，0.5mM， 1mM and 2mM for 24hrs or 48hrs. After treatments， the hygrochastic seeds were planted apart. Then counted the germination rates of seeds and observed if there was mutation. The germination rate of hygrachastic seeds treated with 0， 0.5， 1， 2mM NaN3 for 24hrs were 1.43%， 10%，22.86% and 11.76%. After 48hr， the germination rate were 0%， 0%， 1.52%，12.12%. There have been three mutated plants induced by NaN3 treatment.One is with leaf variegation along the central vines and the others are with thinner and undulated leaves. As to callus induction， medium with 12，4-D 1mg/L had the best effect on callus induction， and 2，4-D 1mg/L caused browning frequently. Under the other treatments， explants grew greenly and had less calli. Most of calli formed from the cut places of explants， and were friable without embro-like body. Calli subcultured in medium with 2，4-D and 2ip can keep growing， but still without embryo-like body.