The purpose of this plan is to promote the plant pathogens detection and isolation efficiency of the green house.This year we promoted the function of the two plant pathogens detection and isolation green horses to fit in the certification requirements of the virus detection (viruses which infect the crop include orchid， potato and asparagus bean) of the Taiwan Accreditation Foundation. The most common way of Genetically Modified (GM) soybean inspecting method is Polymerase Chain Reaction (PCR) inspecting method， to amplify the sample target DNA fragment.Establish the SOP of GM soybean (Roundup Ready， GTS 40-3-2) qualitative detection method and passed the ISO / IEC 17025 accreditation. The main detection methods of cruciferous black rot bacteria (Xanthomonas campestris pv. campestris) are selective medium culturing， SDS immunodiffusion， ELISA， and Biolog rapid identification system. For watermelon bacterial fruit blotch (Acidovorax avenae subsp. Citrulli)，the detection methods are symptoms diagnosis， tissue examination，pathogen isolation and identification， PCR with specific primer sets，serum-based diagnostic， and selective medium culturing. The detection methods will be tested and then developed into a standardized screening process.
The purpose of this plan is to promote the plant pathogens detection and isolation efficiency of the green house.This year we promoted the function of the two plant pathogens detection and isolation green horses to fit in the certification requirements of the virus detection (viruses which infect the crop include orchid， potato and asparagus bean) of the Taiwan Accreditation Foundation. The most common way of Genetically Modified (GM) soybean inspecting method is Polymerase Chain Reaction (PCR) inspecting method， to amplify the sample target DNA fragment.Establish the SOP of GM soybean (Roundup Ready， GTS 40-3-2) qualitative detection method and passed the ISO / IEC 17025 accreditation. The main detection methods of cruciferous black rot bacteria (Xanthomonas campestris pv. campestris) are selective medium culturing， SDS immunodiffusion， ELISA， and Biolog rapid identification system. For watermelon bacterial fruit blotch (Acidovorax avenae subsp. Citrulli)，the detection methods are symptoms diagnosis， tissue examination，pathogen isolation and identification， PCR with specific primer sets，serum-based diagnostic， and selective medium culturing. The detection methods will be tested and then developed into a standardized screening process.